Journal: Nature Communications

Article Title: Periodic formation of epithelial somites from human pluripotent stem cells

doi: 10.1038/s41467-022-29967-1

Figure Lengend Snippet: a, b Time-lapse images of the HES7 promoter-luciferase reporter activity in a representative somitoid. The starting point on day 4 was defined as time 0. See also Supplementary Movie . Scale bars: 500 µm. Images were taken by an LV200 luminescent microscope. b Enlarged images of several time points are also shown. Arrows indicate the somite boundaries. BF: Bright field. c Kymograph of the HES7 reporter activity measured along the posterior-anterior axis (the white arrow) of the same sample shown in a , b . d Detrended intensity (top) and the oscillation phase (bottom) of the HES7 reporter activity in the same sample shown in a , b . Blue and orange lines indicate the signals measured in the posterior and anterior regions of somitoids, respectively, marked in c . The gaps in the graph correspond to short halts of imaging to adjust the sample position. N = 14 samples showed similar oscillatory patterns. e Relationship between HES7 oscillations and segmentation timings. Detrended HES7 reporter activity of the entire image of the same sample shown in a and b. Arrows indicate the timings of somite formation, and the colors correspond to the arrows in b . f Periods calculated from the HES7 oscillations and somite formation. Mean ± SEM. Each point in the graph indicates an average period of several oscillation peaks or somite formation timings in one sample from day 5 to day 6. N = 14 (HES7 oscillation) and 17 (Somite formation). 5 independent experiments. P -value is from two-sided student’s t-test. Source data are provided as a Source Data file.

Article Snippet: Live-luminescence imaging was performed with an LV200 luminescent microscope (Olympus, cellSens Dimension software) using a 10x objective lens (UPLXAPO10X).

Techniques: Luciferase, Activity Assay, Microscopy, Imaging

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Morphologic profiling of Sulfasalazine, DHA and the novel hybrid compound AC254 on rodent glioma cells. ( A ) Rat glioma cells (F98) were treated with a range of concentrations of SAS, DHA, AC254, SAS and solvent DMSO combined with DHA. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µM. ( B ) F98 were treated with 0.5 µM of SAS, DHA, AC254 and SAS combined with. Cells were fixated and stained with actin marker Phalloidin 488 and DNA marker Hoechst after 24 h of treatment. Axio Observer with Zen Software (Zeiss). Scale bar: 20 µm.

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software, Staining, Marker

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Effects on human cell viability (U87, TN22). ( A ) Cells (U87) were treated with 1 µM of the compounds. Cell viability was measured after 72 h. n = 4. * indicates significance to control ( p < 0.05). ( B ) U87 were treated with various concentrations of the compounds. Cell viability was measured the same way as in A. n = 4. * indicates significance to control ( p < 0.05). ( C ) TN22 were treated with 5 µM of the compounds. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µm. ( D ) TN22 were treated with 5 µM of the compounds, cell viability was measured after 72 h. n = 4. * indicates significance to control ( p < 0.05).

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Impact on human cell morphology. ( A ) Human cells (U87) were treated with a variation of concentrations of SAS, DHA, their covalently bound hybrid (AC254) and their 1:1 mixture. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µM. ( B ) Cells (U87) were treated with 5 µM of SAS, DHA, AC254 and SAS combined with. Cells were fixated and stained with actin marker Phalloidin 488 and DNA marker Hoechst after 24 h of treatment. Axio Observer with Zen Software (Zeiss). Scale bars represents 20 µm.

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software, Staining, Marker

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Impact of the compounds on cell migration. ( A , B , C ) A scratch was made into plated cells (F98) after 36 h. Cells were then treated with 0.5 µM ( A ) and 5 µM ( B , C ) of the compounds. Representative images were taken (Olympus × 71 with cell F-Software (Olympus)). ( B ), quantified and related to the time the scratch was made (0 h) ( A , C ). n = 3. * indicates significance to control ( p < 0.05).

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Migration, Software